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This document is intended for epidemiologists, statisticians and laboratory technicians responsible for the use of HIV incidence assays for surveillance and epidemic monitoring purposes. People that are not technical experts in this field may prefer to first read the UNAIDS/WHO Working Group on Global HIV/AIDS and STI Surveillance published guidelines on When and How to Use Assays for Recent Infection to Estimate HIV Incidence at a Population Level for a basic introduction to this topic.