After more than 30 years of the HIV epidemic—and an unprecedented medical and social response—discrimination, violence, harmful gender norms and related stigma continue to pose challenges to an effective HIV response. The findings of these assessments indicate that gender-related barriers pose significant obstacles to the uptake of services that prevent new HIV infections among children and keep mothers alive—obstacles that require urgent attention. Without dedicated attempts to overcome these gender-related barriers, current efforts will meet with limited success, and the needs and rights of both women and children will remain compromised.